adi spa 835 d rabbit anti stip1 proteintech  (Proteintech)

Journal: EMBO Reports

Article Title: Kinesin-1 mediates proper ER folding of the Ca V 1.2 channel and maintains mouse glucose homeostasis

doi: 10.1038/s44319-024-00246-y

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-STIP1 , Proteintech , Cat # 15218-1-AP.

Techniques: Knock-Out, Recombinant, Plasmid Preparation, Knockdown, Expressing, Transduction, Sequencing, Software, Enzyme-linked Immunosorbent Assay, Blocking Assay, Transfection, Isolation, Western Blot

Journal: Journal of Cellular and Molecular Medicine

Article Title: Stress‐induced phosphoprotein 1 restrains spinal cord ischaemia‐reperfusion injury by modulating NF‐κB signalling

doi: 10.1111/jcmm.17030

Figure Lengend Snippet: Primary antibodies used for Western blotting assay

Article Snippet: Rabbit anti‐human STIP1 monoclonal antibody , 1:1,000 , A0036 , ABclonal, Inc., Wuhan, China.

Techniques: Western Blot

Journal: Genes, chromosomes & cancer

Article Title: Expression of Stress-Induced Phosphoprotein1 (STIP1) is Associated with Tumor Progression and Poor Prognosis in Epithelial Ovarian Cancer

doi: 10.1002/gcc.22136

Figure Lengend Snippet: STIP1 is highly expressed in human ovarian cancer cells and tissue specimens. A. STIP1 protein levels were analyzed by western blot. GAPDH was included as an internal loading control. B. Representative immunohistochemical staining for STIP1 in formalin-fixed paraffin-embedded EOC tissues (400×). C. IHC staining score of STIP1 in EOC samples was significantly higher than that in healthy controls, benign ovarian tumors, or borderline ovarian tumors (P < 0.001, P < 0.001, and P = 0.039 respectively). Differences between tumor stages and grades were statistically significant, with advanced-stage and poor-grade specimens having higher immunoreactivity.

Article Snippet: The membranes were blocked with 5% skim milk in TBST (50 mM Tris, 150 mM NaCl, 0.05% Tween-20, pH 7.5) for 1 h at room temperature, followed by incubation with anti-STIP1 rabbit polyclonal antibody (mouse monoclonal, Cat.# sc-32761, Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad5 (Cell Signaling, Danver, MA), anti-phosphoSmad1/5(Ser463/465) (Cell Signaling), anti-human β-actin (Cell Signaling), or anti-GAPDH (Santa Cruz Biotechnology) overnight at 4°C.

Techniques: Western Blot, Control, Immunohistochemical staining, Staining, Formalin-fixed Paraffin-Embedded, Immunohistochemistry

Journal: Genes, chromosomes & cancer

Article Title: Expression of Stress-Induced Phosphoprotein1 (STIP1) is Associated with Tumor Progression and Poor Prognosis in Epithelial Ovarian Cancer

doi: 10.1002/gcc.22136

Figure Lengend Snippet: Expression of STIP1 in Relation to Clinicopathological Characteristics in IHC Analysis

Article Snippet: The membranes were blocked with 5% skim milk in TBST (50 mM Tris, 150 mM NaCl, 0.05% Tween-20, pH 7.5) for 1 h at room temperature, followed by incubation with anti-STIP1 rabbit polyclonal antibody (mouse monoclonal, Cat.# sc-32761, Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad5 (Cell Signaling, Danver, MA), anti-phosphoSmad1/5(Ser463/465) (Cell Signaling), anti-human β-actin (Cell Signaling), or anti-GAPDH (Santa Cruz Biotechnology) overnight at 4°C.

Techniques: Expressing, Diagnostic Assay

Journal: Genes, chromosomes & cancer

Article Title: Expression of Stress-Induced Phosphoprotein1 (STIP1) is Associated with Tumor Progression and Poor Prognosis in Epithelial Ovarian Cancer

doi: 10.1002/gcc.22136

Figure Lengend Snippet: Kaplan-Meier plots for patients with EOC stratified according to tumor stage, STIP1 expression, tumor grade, or CA125 expression.

Article Snippet: The membranes were blocked with 5% skim milk in TBST (50 mM Tris, 150 mM NaCl, 0.05% Tween-20, pH 7.5) for 1 h at room temperature, followed by incubation with anti-STIP1 rabbit polyclonal antibody (mouse monoclonal, Cat.# sc-32761, Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad5 (Cell Signaling, Danver, MA), anti-phosphoSmad1/5(Ser463/465) (Cell Signaling), anti-human β-actin (Cell Signaling), or anti-GAPDH (Santa Cruz Biotechnology) overnight at 4°C.

Techniques: Expressing

Journal: Genes, chromosomes & cancer

Article Title: Expression of Stress-Induced Phosphoprotein1 (STIP1) is Associated with Tumor Progression and Poor Prognosis in Epithelial Ovarian Cancer

doi: 10.1002/gcc.22136

Figure Lengend Snippet: Univariate and Multivariate Analyses of the Associations Between Prognostic Variables and Overall Survival in 113 Cases of Epithelial Ovarian Cancer

Article Snippet: The membranes were blocked with 5% skim milk in TBST (50 mM Tris, 150 mM NaCl, 0.05% Tween-20, pH 7.5) for 1 h at room temperature, followed by incubation with anti-STIP1 rabbit polyclonal antibody (mouse monoclonal, Cat.# sc-32761, Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad5 (Cell Signaling, Danver, MA), anti-phosphoSmad1/5(Ser463/465) (Cell Signaling), anti-human β-actin (Cell Signaling), or anti-GAPDH (Santa Cruz Biotechnology) overnight at 4°C.

Techniques:

Journal: Genes, chromosomes & cancer

Article Title: Expression of Stress-Induced Phosphoprotein1 (STIP1) is Associated with Tumor Progression and Poor Prognosis in Epithelial Ovarian Cancer

doi: 10.1002/gcc.22136

Figure Lengend Snippet: STIP1 knockdown by siRNA in EOC cells. Whole cell lysates and total RNA were collected from SKOV3, OVCAR3, or YDOV-13 cells following STIP1 knockdown by siRNA. Expression of STIP1 mRNA was measured by real-time PCR (A) and protein level was analyzed by immunoblotting (B). β-actin mRNA levels were used to normalize STIP1 mRNA expression. An asterisk (*) indicates a P value <0.05.

Article Snippet: The membranes were blocked with 5% skim milk in TBST (50 mM Tris, 150 mM NaCl, 0.05% Tween-20, pH 7.5) for 1 h at room temperature, followed by incubation with anti-STIP1 rabbit polyclonal antibody (mouse monoclonal, Cat.# sc-32761, Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad5 (Cell Signaling, Danver, MA), anti-phosphoSmad1/5(Ser463/465) (Cell Signaling), anti-human β-actin (Cell Signaling), or anti-GAPDH (Santa Cruz Biotechnology) overnight at 4°C.

Techniques: Knockdown, Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Genes, chromosomes & cancer

Article Title: Expression of Stress-Induced Phosphoprotein1 (STIP1) is Associated with Tumor Progression and Poor Prognosis in Epithelial Ovarian Cancer

doi: 10.1002/gcc.22136

Figure Lengend Snippet: Effects of STIP1 on cell proliferation, migration, and invasiveness of EOC cells. A. Cell proliferation of SKOV3, OVCAR3, and YDOV-13 was determined by MTT assay 72 h after siRNA transfection. Error bars represent the SD of triplicate experiments. Cell migration (B) and invasion (C) analyses of SKOV3 cells were performed. Migrating or invasive cells were quantified using a plate reader at OD 560 nm. D. STIP1 knockdown (siSTIP1#1 and siSTIP1#2) decreased mRNA level of ID3 in OVCAR3 cells. E. OVCAR3 cells were assayed for phospho-Smad1/Smad5. STIP1 knockdown (siSTIP1#1 and siSTIP1#2) reduced phosphorylation of Smad1/Smad5. The total amount of Smad5 and actin was used as the protein loading control. An asterisk (*) indicates a P value <0.05.

Article Snippet: The membranes were blocked with 5% skim milk in TBST (50 mM Tris, 150 mM NaCl, 0.05% Tween-20, pH 7.5) for 1 h at room temperature, followed by incubation with anti-STIP1 rabbit polyclonal antibody (mouse monoclonal, Cat.# sc-32761, Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad5 (Cell Signaling, Danver, MA), anti-phosphoSmad1/5(Ser463/465) (Cell Signaling), anti-human β-actin (Cell Signaling), or anti-GAPDH (Santa Cruz Biotechnology) overnight at 4°C.

Techniques: Migration, MTT Assay, Transfection, Knockdown, Phospho-proteomics, Control

Journal: Genes, chromosomes & cancer

Article Title: Expression of Stress-Induced Phosphoprotein1 (STIP1) is Associated with Tumor Progression and Poor Prognosis in Epithelial Ovarian Cancer

doi: 10.1002/gcc.22136

Figure Lengend Snippet: STIP1 knockdown inhibits in vivo xenograft tumor growth. For the xenograft tumor growth assay, treatments were started 1 week after tumor cell injection, and siControl or siSTIP1 was injected every 3 days for 3 weeks at a dose of 5 mg/kg body weight. A. STIP1 knockdown reduces the growth rate of xenografted SKOV3 cells in BALB/c-nu mice. Mean tumor volume ± SD for each group was calculated at each week. B. Ki-67, BCL2, Tp53, and Bax mRNA levels were analyzed by qRT-PCR and normalized to β-actin mRNA levels. An asterisk (*) indicates a P value <0.05.

Article Snippet: The membranes were blocked with 5% skim milk in TBST (50 mM Tris, 150 mM NaCl, 0.05% Tween-20, pH 7.5) for 1 h at room temperature, followed by incubation with anti-STIP1 rabbit polyclonal antibody (mouse monoclonal, Cat.# sc-32761, Santa Cruz Biotechnology, Santa Cruz, CA), anti-Smad5 (Cell Signaling, Danver, MA), anti-phosphoSmad1/5(Ser463/465) (Cell Signaling), anti-human β-actin (Cell Signaling), or anti-GAPDH (Santa Cruz Biotechnology) overnight at 4°C.

Techniques: Knockdown, In Vivo, Growth Assay, Injection, Quantitative RT-PCR